Detection and persistence of unique TCRs over time using TCR sequencing. (A-C) Shown are unique clonotypes that were present in the VST cell product but not present before VST cell infusion. (D) TCR-Vβ diversity over time in patient P2891 in CD45RA⁺ (naïve) and CD45RA^– (memory) T-cell populations. (E) Persistence of unique T-cell clones over time in all patients with available data. (F) Unique T-cell clone contribution at 1 month after VST infusion separated by whether patients had detectable virus within the first month of infusion (reactivation) or not (no reactivation).