Expression of several miRNA candidates associated with the downregulation of endothelial-ligand expression. (A) Data represent the numbers of miRNAs detected in RA^– and RA⁺ T exosomes. (B) Several representative miRNAs were validated for their upregulated expression in RA⁺ T exosomes by using a qPCR assay. U6 was used as an endogenous reference gene for normalizing miRNA levels. Data from qPCR analysis were normalized to the controls that represented samples from the RA^– T exosomes after being normalized to a reference U6 gene (2^-ddCT). Data are presented as the mean ± SEM obtained from 3 or 4 independent experiments. *P = .01 to <.05; **P = .001 to <.01 (vs RA^–).