Distribution of somatic mutations in immunophenotypically distinct subpopulations in de novo MPAL. (A) An example of MPAL with T/B/M differentiation (patient 14 in Figure 2; B-blast component is not shown). (B) An example of MPAL with T/B differentiation (patient 16 in Figure 2). (C-D) An example of T/B/M MPAL by flow cytometry (patient 17). (E-G) VAFs of various mutated genes in sorted blast populations (E, patient 14; F, patient 16; G, patient 17). Lym, sorted natural killer cell population serving as germline reference.