T-cell clonality across 63 samples referred for clinical TCR clonality testing. Boxes represent individual unique VJ rearrangements, and box size reflects abundance in sample. BIOMED-2 clonality assessments are indicated as green (clonal), red (polyclonal), or yellow (not performed). CapTCR-seq binary clonality calls are indicated as green (enriched for a single rearrangement) or red (nonenriched). CapTCR-seq continuous Clonality Scores (supplemental Table 11) are indicated as a gradient from dark green (1) to dark red (0) through white (median), where larger values indicate greater fractional prevalence. Samples are ordered left to right in terms of increasing CapTCR-Seq clonality with an asterisk indicating disagreement between CapTCR-Seq and BIOMED-2 assessments. (A) β chain VJ rearrangements. (B) γ chain VJ rearrangements. Note that samples occur in different orders between the 2 panels to facilitate comparison of CapTCR-seq and BIOMED-2, resulting in the 2 independent loci.