Bone marrow B-cell development and generation of peripheral B-cell subsets is normal in C481S-mutant animals. (A-C, left) Gating strategy for identification of B-cell developmental stages and peripheral subsets in indicated organs. Prior gating is indicated above the FACS plots. Propidium iodide (PI) was used for dead cell discrimination. (A-B, right) Absolute numbers of total B cells and B-cell subsets in bone marrow (BM) and spleen. (C, right) Frequency of subsets within B cells in the peritoneal cavity (PeC). (A-C, right) Each dot represents data from individual animals; vertical bars indicate mean and 2-tailed Mann-Whitney U test was used to calculate significance. FoB, follicular B cell; immB, immature B cell; matB, mature B cell; MZ, marginal zone B cell; Sw, switched B cell; T1, transitional 1 B cell; T2, transitional 2 B cell.