Mn porphyrins alleviate RBC and tissue oxidative stress, and endothelial activation in TS mice in vivo. TS mice were dosed subcutaneously daily for 28 days except on weekends with 1 dose per day of vehicle, 0.1 mg/kg MnBuOE, and 1 mg/kg MnE. (A-C) Quantitative analysis of images of tissue organs (n = 5 random fields): kidneys (A), liver (B), and spleen (C) collected from treated TS mice (n = 4 per treatment condition) stained for ROS with DCF. Graphs show mean fluorescence (±SEM) of data for each organ collected from treated mice; P < .05 for Mn porphyrin–treated vs vehicle-treated (within an organ). (D-F) Mn porphyrin–modulated mRNA expression of ICAM-1 (D), VCAM-1 (E), and P-selectin (F) in tissue organs in TS mice. Relative quantification of mRNA expression (D-F) in the lungs, kidneys, liver, and spleen are presented as the mean ± SEM (n = 4 per organ/treatment condition). (G-J) Western blot analysis shows downregulation of ICAM-1, VCAM-1, and P-selectin expression in the lungs and/or kidneys. Data are presented as the mean ± SEM (n = 6-9 per organ/treatment condition). *P < .05, **P < .01, ***P < .001, and ****P < .0001 vs vehicle treatment. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.