Analysis of vector integration sites in HSPCs. Genomic DNA isolated from 20 pooled GFP⁺ progenitor colonies from BM cells of female hCD46tg animals, harvested 8 weeks after HD-Ad–GFP + HD-Ad–SB in vivo transduction and SB-mediated transgene integration sites were recovered. (A) Chromosomal distribution of integration sites in GFP⁺ CFU colonies. (B) Percentage of total integration events per chromosome. (C) Integration sites were mapped to the mouse genome, and their location with respect to genes was analyzed. Shown is the percentage of integration events that occurred outside of genes, within intronic regions, and within exons, respectively.