Figure 5.
Platelets PS limits thrombosis propensity in the vena cava, but not in the carotid artery. (A) Evaluation of the thrombus volume in the vena cava 30 minutes after the FeCl3 injury, using ultra-high-frequency US (n = 4/genotype). Using the Vevo 3100 imaging system (Fujifilm VisualSonics Canada) and a MX550S linear array transducer (bandwidth, 32-55 MHz; center frequency, 40 MHz), 3-dimensional image series were acquired after 30 minutes of thrombus induction at 76-µm intervals. Using the VevoLab software package (Fujifilm VisualSonics), the 3-dimensional image series were used to make a 3-dimensional reconstruction of the inferior vena cava or carotid artery and a 3-dimensional reconstruction of the thrombus. The vessel and the thrombus area were measured by the software in cubic millimeters. The thrombus volume (%) refers to the volume (in cubic millimeters) that the thrombus occupies inside the volume (mm3) of the vessel. (B) Thrombi collected at the end of the experiment (=30 minutes after the FeCl3 injury) and processed for immunohistochemistry; the sections were performed in the middle of the vena cava thrombus, as shown. Hematoxylin and eosin staining (HE) and immunohistochemistry for insoluble fibrin, PS, and CD31 were realized in Pros1lox/loxPf4Cre− and Pros1lox/loxPf4Cre+ vena cava thrombi 30 minutes after the FeCl3 injury. Scale bar, 500 µm. (C) Evaluation of the thrombus volume in the carotid artery 30 minutes after the FeCl3 injury, using ultrahigh-frequency US (n = 3/genotype). For the method of evaluation of the thrombus volume, see panel A. (D) HE and immunohistochemistry for insoluble fibrin, PS, and CD31 were realized in Pros1lox/loxPf4Cre− and Pros1lox/loxPf4Cre+ carotid artery thrombi 30 minutes after the FeCl3 injury. Scale bar, 200 µm.

Platelets PS limits thrombosis propensity in the vena cava, but not in the carotid artery. (A) Evaluation of the thrombus volume in the vena cava 30 minutes after the FeCl3 injury, using ultra-high-frequency US (n = 4/genotype). Using the Vevo 3100 imaging system (Fujifilm VisualSonics Canada) and a MX550S linear array transducer (bandwidth, 32-55 MHz; center frequency, 40 MHz), 3-dimensional image series were acquired after 30 minutes of thrombus induction at 76-µm intervals. Using the VevoLab software package (Fujifilm VisualSonics), the 3-dimensional image series were used to make a 3-dimensional reconstruction of the inferior vena cava or carotid artery and a 3-dimensional reconstruction of the thrombus. The vessel and the thrombus area were measured by the software in cubic millimeters. The thrombus volume (%) refers to the volume (in cubic millimeters) that the thrombus occupies inside the volume (mm3) of the vessel. (B) Thrombi collected at the end of the experiment (=30 minutes after the FeCl3 injury) and processed for immunohistochemistry; the sections were performed in the middle of the vena cava thrombus, as shown. Hematoxylin and eosin staining (HE) and immunohistochemistry for insoluble fibrin, PS, and CD31 were realized in Pros1lox/loxPf4Cre− and Pros1lox/loxPf4Cre+ vena cava thrombi 30 minutes after the FeCl3 injury. Scale bar, 500 µm. (C) Evaluation of the thrombus volume in the carotid artery 30 minutes after the FeCl3 injury, using ultrahigh-frequency US (n = 3/genotype). For the method of evaluation of the thrombus volume, see panel A. (D) HE and immunohistochemistry for insoluble fibrin, PS, and CD31 were realized in Pros1lox/loxPf4Cre− and Pros1lox/loxPf4Cre+ carotid artery thrombi 30 minutes after the FeCl3 injury. Scale bar, 200 µm.

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