Known HbF regulators are not differentially expressed in CD34⁺ HSPC-derived Ferythroblasts. (A) RNA-seq transcript levels of selected known HbF regulators. Data are shown as average normalized reads from 2 biological replicates. (B) RT-PCR analysis of BCL11A and LRF expression from sorted day 11 F and A cells from a total of 9 donors across 3 experiments. Data are shown relative to A cells as mean ± SD. (C) Western blot analysis of HbF regulators from sorted day 11 F and A cells from 3 separate donors. (D) RT-PCR analysis of NuRD complex components from sorted day 11 F and A cells from a total of 9 donors across 3 experiments. Data are shown relative to A cells as mean ± SD.