Diminished APC and mDia1 expression in DKO MKs, but not platelets. mRNA content of Apc (A) and Diaph1 (B) in in vitro-matured MKs was quantified by quantitative polymerase chain reaction. Levels were normalized to Sdha and Actb. Values are mean ± SD (n = 3). Unpaired, 2-tailed Student t test. *P < .05. (C-D) MKs or washed platelets from WT, Twf1^−/−, Cof1^−/−, or DKO mice were immunoblotted for the cytoskeletal regulatory proteins APC, mDia1, and RhoA (D). Images were acquired using an Amersham Image 680 (GE Healthcare). Blots are representative of 3 independent experiments. (C) Densitometric analysis was performed using ImageJ Software. Values were normalized to WT levels. Values are mean ± SD. Unpaired, 2-tailed Student t test. **P < .01.