Figure 1.
Severe macrothrombocytopenia in MK-specific Twf1/Cof1-deficient mice. (A) Absence of Twf1 and Cof1 was confirmed in platelets derived from WT, Twf1−/−, Cof1−/−, and DKO mice by immunoblotting. Platelet count (B) and size (C) were determined using an automated blood cell analyzer (ScilVet). Values of Twf1−/−, Cof1−/−, and DKO mice were normalized to the respective WT control. Values for all controls are shown. Mean ± SD (n = 9). One-way ANOVA with Sidak correction for multiple comparisons. #P < .05; **P < .01; ***P < .001. (D) Transmission electron microscopic images of WT and DKO platelets. Scale bars, 0.5 µm. Insets display microtubule coils. Scale bars, 0.2 µm. Analysis of microtubule (E), dense granule (F), and α-granule (G) numbers in WT and DKO platelets. Values are mean ± SD (n = 3).

Severe macrothrombocytopenia in MK-specific Twf1/Cof1-deficient mice. (A) Absence of Twf1 and Cof1 was confirmed in platelets derived from WT, Twf1−/−, Cof1−/−, and DKO mice by immunoblotting. Platelet count (B) and size (C) were determined using an automated blood cell analyzer (ScilVet). Values of Twf1−/−, Cof1−/−, and DKO mice were normalized to the respective WT control. Values for all controls are shown. Mean ± SD (n = 9). One-way ANOVA with Sidak correction for multiple comparisons. #P < .05; **P < .01; ***P < .001. (D) Transmission electron microscopic images of WT and DKO platelets. Scale bars, 0.5 µm. Insets display microtubule coils. Scale bars, 0.2 µm. Analysis of microtubule (E), dense granule (F), and α-granule (G) numbers in WT and DKO platelets. Values are mean ± SD (n = 3).

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