Circulating Tfh subsets are altered in WAS patients. (A) Flow cytometric analysis of CD4⁺CXCR5⁺ T-cell subsets in WAS patients and HCs. (B) Percentage of CD4⁺CXCR5⁺ T-cell subsets in WAS patients (n = 24; P1-P24) and HCs (n = 24). (C) Percentage of CD4⁺CXCR5⁺ T-cell subsets in WAS patients with clinical scores of 3 to 4, 5A and age-matched HCs. (D) Percentage of CD4⁺CXCR5⁺ T-cell subsets in WASp-positive, WASp-negative, and WASp double-peak patients and age-matched HCs. (E) Ratio of CXCR5⁺ (Th2 + Th17)/Th1 cells. (F) Ratio of CXCR5⁺(Th2 + Th17)/Th1 cells in WAS patients with clinical scores of 3 to 4, 5A and age-matched HCs. (G) Ratio of CXCR5⁺(Th2 + Th17)/Th1 cells in WASp-positive (WASP+), WASp-negative (WASp−), and WASp double-peak patients (WASp-DP) and age-matched HCs. (H) Percentages of Th1-like, Th2-like, and Th17-like Tfh cells from spleen of WT and WAS KO mice. (I) Ratio of CXCR5⁺(Th2 + Th17)/Th1 cells from spleen of WT and WAS KO mice.