WAS patients have fewer CXCR5⁺ CD4⁺ T cells in blood. (A) Flow cytometric analysis of CD4⁺CXCR5⁺ T cells in WAS patients and HCs. (B) Percentage and number of CXCR5⁺ cells within total CD4⁺ T cells in WAS patients (n = 35) and HCs (n = 35). (C) Percentage of CD45RA⁻ memory cells within total CD4⁺ T cells in WAS patients (n = 24; patient 1 [P1]-P24) and HCs (n = 24). (D) Percentage and number of CD4⁺ T cells in WAS patients (n = 35) and HCs (n = 35). (E) Percentage of CXCR5⁺ cells within total CD4⁺ T cells at different ages. (F) Percentage of CXCR5⁺ cells within CD45RA⁻ memory CD4⁺ T cells in WAS patients (n = 24; P1-P24) and HCs (n = 24). (G) Percentage of CXCR5⁺ cells within CD45RA⁻ memory CD4⁺ T cells at different ages. Linear regression analyses in HCs or WAS patients are indicated by dotted lines and solid lines, respectively. The 95% confidence interval for each is indicated by the neighboring thinner dotted lines. (H) Percentages of CXCR5⁺ cells within CD4⁺ T cells in WAS patients with clinical scores of 3 to 4, 5A and age-matched HCs. (I) Percentages of CXCR5⁺ cells within CD4⁺ T cells in WASp-positive (WASP+), WASp-negative (WASP−), and WASp double-peak patients (WASP-DP) and age-matched HCs.