Flow cytometry plot for patient 2. T cells from CSF of patient 2 at day 8 postinfusion were predominantly CD4⁺. Cells isolated from CSF were stained with a combination of CD3, CD4, CD8, and CD45 and analyzed by flow cytometry. The cells were gated on lymphocytes, the lymphocytes were analyzed for CD3 expression (left panel), and the gated CD3 cells were analyzed for the presence of CD4 and CD8 (right panel).