American Society of Hematology

Figure 1.

$Methylated EBV DNA from latently infected cells is preferentially enriched by binding to methyl-DNA (MBD2) binding beads. (A) Schematic presentation of the technique. DNA is incubated with methyl-DNA binding beads. DNA from the unbound (noncaptured) fraction, washes (300 and 400 mM NaCl), and eluate (2000 mM NaCl elution) are all quantitatively amplified by quantitative PCR. (B) EBV methylation index of DNA from virions, a lymphoblastoid cell line, and Burkitt cell lines Raji, Namalwa, and Akata. (C) EBV methylation index of Akata cells in the absence of lytic replication, and after incubation with 50 μg/mL anti-human immunoglobulin G (IgG) to induce lytic viral replication.$

Methylated EBV DNA from latently infected cells is preferentially enriched by binding to methyl-DNA (MBD2) binding beads. (A) Schematic presentation of the technique. DNA is incubated with methyl-DNA binding beads. DNA from the unbound (noncaptured) fraction, washes (300 and 400 mM NaCl), and eluate (2000 mM NaCl elution) are all quantitatively amplified by quantitative PCR. (B) EBV methylation index of DNA from virions, a lymphoblastoid cell line, and Burkitt cell lines Raji, Namalwa, and Akata. (C) EBV methylation index of Akata cells in the absence of lytic replication, and after incubation with 50 μg/mL anti-human immunoglobulin G (IgG) to induce lytic viral replication.

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