Impaired production of effector cytokines and degranulation in CLL-derived Vγ9Vδ2-T cells. (A) Production of IFN-γ, TNF-α, IL-17 (CLL, n = 14; HC, n = 12), and IL-4 (CLL and HC, n = 4) by Vγ9Vδ2-T cells after stimulation with PMA/ionomycin for 4 hours. (B) CD19-depleted PBMCs from CLL patients (n = 8) or HCs (n = 8) were cocultured with allogeneic CLL cells or Daudi cells for 16 to 18 hours. During the last 6 hours of coculture, brefeldin A and GolgiStop were added to measure cytokine production in Vγ9Vδ2-T cells. (C) CD107a expression in Vγ9Vδ2-T cells after PMA/ionomycin stimulation for 4 hours (CLL, n = 14; HC, n = 12). (D) CD107a expression after coculture with malignant B cells as in (B). Data are mean and SEM. *P < .05, 1-way ANOVA, followed by the Bonferroni post hoc test (A), Student t test (B-D).