Frequency and immune phenotype of Vγ9Vδ2-T cells in CLL patients and HCs. Immunophenotyping of Vγ9Vδ2-T cells from untreated CLL patients and age-matched HCs, directly ex vivo. (A) Frequency of Vγ9⁺Vδ2⁺ cells within CD3⁺ T lymphocytes (CLL, n = 39; HC, n = 20). (B) Absolute number of Vγ9Vδ2-T cells (CLL, n = 39; HC, n = 6). (C) Distribution of differentiation subsets within Vγ9Vδ2-T cells based on CD27 and CD45RA expression (CLL, n = 39; HC, n = 20). (D) Expression of exhaustion markers on Vγ9Vδ2-T cells. Geometric mean fluorescence intensity (gMFI) of PD-1 and BTLA expression (CLL, n = 39; HC, n = 20) and CD244, CD160, and LAG-3 expression (CLL, n = 16; HC, n = 11). (E) Frequency of granzyme B⁺ and granzyme K⁺ cells within Vγ9Vδ2-T cells (CLL, n = 7; HC, n = 7). Data are mean and SEM. *P < .05, Student t test. T_CM, CD27⁺CD45RA⁻; T_EM, CD27⁻CD45RA⁻; T_EMRA, CD27⁻CD45RA⁺; T_N, CD27⁺CD45RA⁺.