Effect of rD′D3-FP on FeCl₃-induced arterial occlusion in FVIII-ko and NMRI mice. (A) The effect of rVIII-SingleChain (100 IU/kg, red line), alone or coadministered with rD′D3-FP (2.86 mg/kg, blue line), was assessed 1 hour after FeCl₃-induced damage of the endothelium in an arterial occlusion model in FVIII-ko mice (n = 10). A vehicle control (gray line) was tested as negative control (n = 5), remaining at 0% occlusion rate. There was no significant effect of rD′D3-FP on the coagulation restored by the coadministered rVIII-SingleChain, suggesting no negative impact of rD′D3-FP on hemostasis. (B) Arterial occlusion in vehicle-treated NMRI mice (saline, gray line) was compared with that after administration of high-dose rD′D3-FP (250 mg/kg, blue line) 15 minutes after FeCl₃-induced damage of the endothelium in an arterial occlusion model (n = 8-9). There was a significant effect of high-dose rD′D3-FP on coagulation in NMRI mice. P values were calculated using the log-rank Mantel-Cox test.