CAR-37 in vitro activity against T-cell lymphoma and leukemia. (A) CD37 expression on PTCL tumor cell lines. (B) Representative FACS plots from patient-derived samples. (C) CD69 expression and (D) CD107a degranulation of CAR T cells, as evaluated by flow cytometry after 6 hours of coculture with indicated tumor cells at 1:1 E:T ratio. Degranulation is relative to PMA positive control; representative normal donor is shown. Cytotoxic capacity of CAR-37 T cells was measured after overnight coculture with (E) Hut78 and (F) FEPD target cells at a different E:T ratio. The cytotoxicity assay is representative of 3 independent experiments conducted with different healthy donors. Mean ± SEM shown.