WASP and Mst1 deficiency has an impact on the differentiation of FO and GC B cells. Splenic B cells were stained with antibodies specific for surface markers of FO, MZ, T1, T2, and GC B cells. Samples (n = 9) were then analyzed by using flow cytometry. Shown are representative dot plots (A), the average percentages (± standard error of the mean), and numbers of cells of FO and MZ B cells (B-C), T1 B cells (D-E), T2 B cells (F-G), and GC B cells (H-I) extracted from spleen of 3 independent experiments. One-way analysis of variance with the Tukey test was used to perform multiple group comparisons; *P < .01, **P < .001.