Volcanic plot representing differentially expressed genes of MK-2048–treated cells and Venn diagram of microarray results. (A) Volcano plot (fold change by significance) showing the gene expression ratio of the indicated cells after 16-hour treatment with 25 μM MK-2048. The gene expression ratios of the treated cells relative to untreated cells are shown (n = 3). Approximately 20 000 genes were tested. Red indicates significantly differentially expressed genes (P < .01, fold change (FC) >2); blue indicates UPR-related genes (listed in Table 1). The gene expression levels did not change in OATL4 cells. (B) Venn diagram of microarray results. Overlapping circles demonstrating the number of genes significantly differentially expressed by MK-2048 treatment in common, partially in common, or unique to each specific cell line. Blue circle: SLB-1 cells; red circle: ED cell; green circle: KK1 cells. The UPR-related genes are listed.