American Society of Hematology

Figure 4.

$Identification of cell types with increased mTOR activation. (A) Co-IF for CD45, MUM1, CD138, CD3, CD20, and CD68 was performed to identify the cell types expressing pS6 in 4 iMCD-TAFRO cases (CD20 staining was only performed in 3 cases). Representative images (×20) are shown in panels B-E, where pS6 was stained red and the cell specific marker green: (B) CD138, (C) CD3, (D) CD20, (E) CD68. Labeled cells were manually counted to assess for percentage of pS6+ cells. A majority of pS6+ cells were of the hematopoietic lineage (CD45+). Only a small fraction (2% to 10%) of pS6+ cells were T cells (CD3+). A large proportion of cells expressed the transcription factor MUM1, which is expressed on activated B cells, T cells, and plasma cells. Plasma cells (CD138+) and macrophages (CD68+) also made up a large proportion of pS6+ cells.$

Identification of cell types with increased mTOR activation. (A) Co-IF for CD45, MUM1, CD138, CD3, CD20, and CD68 was performed to identify the cell types expressing pS6 in 4 iMCD-TAFRO cases (CD20 staining was only performed in 3 cases). Representative images (×20) are shown in panels B-E, where pS6 was stained red and the cell specific marker green: (B) CD138, (C) CD3, (D) CD20, (E) CD68. Labeled cells were manually counted to assess for percentage of pS6⁺ cells. A majority of pS6⁺ cells were of the hematopoietic lineage (CD45⁺). Only a small fraction (2% to 10%) of pS6⁺ cells were T cells (CD3⁺). A large proportion of cells expressed the transcription factor MUM1, which is expressed on activated B cells, T cells, and plasma cells. Plasma cells (CD138⁺) and macrophages (CD68⁺) also made up a large proportion of pS6⁺ cells.

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