BRGS^humanmice show efficient reconstitution of human solid cancer cells. (A) Tumor growth curves of DLD-1 cells. DLD-1 cells were transplanted subcutaneously into BRGS^NOD or BRGS^human mice, and tumor volume was measured every week (n = 6 each). (B) Analysis of tumor-forming capabilities of DLD-1 cells in BRGS^NOD and BRGS^human recipients using an extreme limiting dilution assay; 40, 200, or 1000 DLD-1 cells were injected subcutaneously into BRGS^NOD and BRGS^human mice (n = 6 each). Tumor formation was examined 4 weeks after inoculation. (C) Growth curves of xenograft tumors produced by patient-derived CRC cells 10 weeks after inoculation (n = 6 each). Data are mean ± standard deviation. (D) The tumor harvested from each mouse at 10 weeks after inoculation. *P < .05, **P < .01.