Intrinsic and extrinsic PIM1 activation can co-occur. (A) HPB-ALL cells were stimulated with 100 ng/mL IL7 for 30 minutes and stained for pSTAT5 (Tyr 694) conjugated to APC (left panel). For downstream differential transcriptional analysis via PIM1 RT-qPCR, cells were stimulated with 50 ng/mL IL7 for 24 hours (right panel). (B) PDX#3 spleen cells were stimulated with 100 ng/mL IL7 for 30 minutes and stained for pSTAT5 (Tyr 694) conjugated to APC (left panel). For downstream differential transcriptional analysis via PIM1 RT-qPCR, cells were stimulated with 50 ng/mL IL7 for 24 hours (right panel). *P < .05; **P < .01