Loss of p62 delays leukemia development. (A) Kaplan-Meier curve represents survival of WT (n = 10) and p62^−/− (n = 15) MN1 leukemic mice. (B) Kaplan-Meier curve represents survival of shCtrl (n = 10) and shp62 (n = 11) Hoxa9/Meis1 leukemic mice. (C) Flow cytometric analysis of Lin⁻ and leukemic common lymphoid progenitor (L-CMP) (cKit⁺Sca1⁻CD34⁺CD16/32⁺) in MN1 leukemic mice at time of death (n = 9 in WT and n = 8 in p62^−/− group). (D) Flow cytometric analysis of Lin⁻ and leukemic granulocyte macrophage progenitor (L-GMP) (cKit⁺Sca1⁻CD34⁺CD16/32⁻) in Hoxa9/Meis1 leukemic mice at time of death (n = 10 in shCtrl and n = 11 in shp62 group). (E) Colony numbers and (F) cell numbers were determined in colony-forming unit assays of p62^−/− and WT MN1-driven ldMBM leukemia cells isolated from the BM of sick mice at time of death (n = 8 in WT and n = 14 in p62^−/− group). Each experiment was performed in triplicate with 500 LK cells. (G) Kaplan-Meier curve represents survival of secondary recipients transplanted with 10⁴ GFP⁺ cells from WT or p62^−/− MN1 leukemic mice (n = 9 in both groups) into sublethally irradiated (5.5 Gy) WT-recipient mice. Values are mean ± SEM. *P ≤ .05; **P ≤ .01; ***P ≤ .001.