VWF HBD-functionalized fibrin matrices enhance GFs retention in vitro and in VWF-deficient mice. (A-B) GF retention in fibrin matrix. Graph showing the cumulative release of (A) VEGF-A165 or (B) PDGF-BB over 5 days (n = 4; data are mean ± SEM). (C-E) Full-thickness back-skin wounds in VWF-deficient mice were treated with fibrin only or fibrin functionalized with α₂PI_1–8-VWF HBD. (C) Five days after the wound treatment, the number of CD31⁺/CD45^– ECs was determined by using flow cytometry (data are mean ± SEM). (D-E) The amounts of (D) VEGF-A and (E) PDGF-BB in the wounds were quantified by ELISA and normalized by the weight of the wound tissue (data are mean ± SEM). (A-B) Mann-Whitney U test for each time point, (C) ANOVA with Tukey’s test, and (D-E) Mann-Whitney U test. **P < .01; *P < .05.