R1341 mutations observed in VWD type 2B patients impaires VWF-GF binding. (A) Binding of VEGF-A165, PDGF-BB, and FGF-2 to VWF A1 HBDs with R1341 substitutions. (n = 4; data are mean ± SD). (B) Binding of VEGF-A165, PDGF-BB, and FGF-2 to recombinant human VWF (rhVWF) with R1341Q substitution (n = 4; data are mean ± SD). ANOVA with (A) Tukey’s test and (B) Mann-Whitney U test. **P < .01; *P < .05.