Comparison of MEDEP and primary cell differentiation. (A) PCA of the proteome evolution in primary cells (red dots) and MEDEP cells (orange dots) at different differentiation stages. Z-score transformation values were used for this analysis. (B-C) Absolute quantification of proteins involved in heme biosynthesis (B) and in iron metabolism (C) in primary erythroid precursors (PC, circles) and MEDEP cells (M, squares) during the differentiation process. (D) Absolute quantification of transcription factors involved in erythroid differentiation during primary cell and MEDEP cell differentiation (same symbols as in panels B and C).