Expression of LAG3 on TILs in DLBCL. Fresh-frozen deaggregated lymph nodes were examined using flow cytometry. (A) Greater percentages of LAG3^high T cells are found in CD4⁺ nTregs compared with other T-cell subsets, with high percentages also observed in CD8⁺ T cells. (B) LAG3^high TILs had almost universal coexpression of PD-1. (C) TILs coexpressing TIM-3 and LAG3 were common but were significantly increased in all subsets compared with non-Treg CD4⁺ T cells. (D) TILs with coexpression of LAG3, TIM-3, and PD-1 were significantly increased in all T-cell subsets compared with non-Treg CD4⁺ T cells. *P < .05, **P < .01. NS, not significant.