Hel-FL or Hel-Δ3B coexpression with FOXP3 alters gene expression and pathway enrichment in CD4⁺or CD8⁺eTregs compared with FOXP3 alone. All comparisons in this figure use FOXP3 eTregs as the baseline for comparison of CD4⁺ or CD8⁺ eTregs as indicated. (A) Volcano plots depicting gene expression differences between the cell strains. Within the volcano plots, genes were colored if they had a false discovery rate (FDR) ≤0.1. Blue denotes downregulation, and red represents upregulation. The 2 vertical lines represent logFC= −2 and logFC= 2. The horizontal line presents –log10(0.05). (B) Summary of normalized enrichment scores (NES) of KEGG pathways with P ≤ .05 that were enriched in the comparison of two eTreg cell strains indicated after GSEA. Blue bars are pathways enriched in the baseline eTregs, and red bars are pathways enriched in eTregs being compared.