OTX P/R sAML cells exhibit a dysregulated transcriptome with enrichment of TCF7L2/LEF1 target genes. (A) mRNA-seq analysis was performed on total RNA from HEL, HEL-OTX P/R, SET-2, and SET-2-OTX P/R cells. The heat map shows the number of up and downregulated genes in the OTX P/R cells compared with the parental cells at a log2 fold change of ≥1.5 and a P value < .05. (B) Venn diagram of overlap in upregulated genes in the HEL-OTX P/R and SET-2-OTX P/R cells. (C) GSEA of HEL-OTX P/R cells with TF target datasets from the Molecular Signatures Database (MSigDB). F.D.R., false discovery rate; N.E.S., normalized enrichment score. All q-values < 0.1. (D) Gene Ontology analysis (MSigDB) of the common upregulated genes in HEL-OTX P/R and SET-2-OTX P/R cells. (E) Relative mRNA expression of selected TCF7L2 target genes in SET-2-OTX P/R and HEL-OTX P/R cells compared with parental cells as determined by qPCR analysis.