The role of VWF in arterial thrombosis in murine models of HIT. (A-B) Rose bengal photochemical injury studies of the carotid artery under the indicated interventions. (C-D) Cremaster laser arteriole injury studies. (A) Time to occlusion (<10% of initial blood flow volume) and (B) AUC of each individual sample relative to the average AUC displaced by mice infused with TRA. AUC indicates the total flow through the vessel in HIT mice induced with KKO with or without exposure to NAC (0.8 mg/g) or ADAMTS13 (0.1 µg/g). Mice infused with TRA served as a negative control. Individual data points are shown. P values were determined by a 2-sided Mann-Whitney t test. (C) Fibrin accumulation and (D) platelet accumulation 15 minutes after antibody infusion ± ADAMTS13 compared with measurements before intervention. N = 3 mice (15 injuries) per arm. Mean and 1 SEM are shown. P values were determined by 1-way ANOVA.