Inhibition of hPF4-VWF-HIT antibody complexes in vitro. Effect of ADAMTS13 or NAC on hPF4-VWF and hPF4-VWF-HIT antibody complexes. (A) Representative images from studies in Figure 3A after infusion of PF4 (25 µg/mL) into each lane. In some studies, KKO (10 µg/mL), ADAMTS13 (0.7 µg/mL), and/or NAC (10 mg/mL) was added as indicated. (B) The number of VWF strings present in the channel after exposure to the indicated solution is expressed relative to those present in channels following injury and exposure to hPF4 + KKO alone. Means ± 1 SEM are shown for N ≥ 5 studies. P values were determined by 1-way ANOVA.