C587R mutant peripheral lymphocytes are sensitized to apoptosis and underrepresented under competitive condition (A) Peripheral lymphocytes carrying the C587R mutation are sensitized to apoptosis. Growth factor deprivation assay of FACS-sorted naive CD4, CD8 T cells and Fo B cells from either C587R/+ or WT animals. Representative experiment of 3. Each point is the average of 3 technical replicates and bar represents standard deviation. (B) The deleterious effect of the C587R mutation on B-cell survival is masked by the deletion of Bax and Bak1. Representative experiment of 2. Each point is the average of 3 technical replicates and bar represents standard deviation. (C) BM competitive transplant assay. (D) Peripheral B- and T-cell compartments are underrepresented in competitive BM chimera. Twelve weeks posttransplant, the relative representation of “test” and “competitor” cells among splenic lymphocytes was assessed. Data show mean with SD. **P < .01; ***P < .001; and ****P < .0001.