Peripheral lymphocytes but not progenitors are decreased in C587R/+ animals. (A) Representative FACS plots of B-cell compartments in the BM of Nxf1^C587R/+ and WT control animals. Gates indicate CD93⁺IgM⁻ progenitor B cells, CD93⁺ IgM⁺ immature B cells, and CD93⁻IgM⁺ mature B cells. CD93⁺IgM⁻CD25⁺ are pro-B/pre-B1 and CD93⁺IgM⁻CD25⁻ are pre-B2. (B) Absolute cell numbers in C587R/+ mutant and control animals. (C) Representative FACS plots of developing T cells in the thymus of Nxf1^C587R/+ and WT control animals. (D) Absolute cell numbers. (E) Representative plots of transitional B cells in the spleen of Nxf1^C587R/+ and WT control animals. Gates indicate IgM^highCD23^low T1, IgM^highCD23⁺ T2, and IgM^lowCD23⁺ T3 transitional B cells. (F) Absolute cell numbers in C587R/+ mutant and control animals. (G) Representative plots of Fo CD21⁺CD23⁺ B cells and CD21^highCD23^low MZ B cells in the spleen of Nxf1^C587R/+ and WT control animals. (H) Fo B cells are decreased mutant animals. Absolute splenic B-cell numbers. (I) Representative plots of CD4 and CD8 T-cell compartments in spleen of Nxf1^C587R/+ and WT control animals. Gates indicate CD62L⁺CD44^low naive, CD62L⁺CD44^high memory and CD62L⁻CD44^high effector T cells. (J) Naive T cells are decreased in C587R/+ mutant animals. Absolute T cells number within the CD4 (J) and CD8 (K) compartments. Circles and triangles represent cell numbers for an individual mouse. Data show mean with SD. *P < .05; **P < .01; and ***P < .001. DP, double positive; IgM, immunoglobulin M; SP, single positive.