LSCI showed gradual recovery of blood flow in the peripheral ischemic area from 1 day after RB-PTS or T+RB-PTS. (A) The CBF was sequentially measured by LSCI before and after photothrombosis plus ligation of the ipsilateral common carotid artery (from 5 hours to 7 days postphotoactivation), and quantified as the percentage to contralateral hemisphere at each time. Shown are the representative LSCI images at various points after RB-PTS and T+RB-PTS (n = 7 for each group). Also shown are the MCA-suppling territory for CBF measurement (3 mm × 4.8 mm) in both hemispheres. Asterisk indicates the site of proximal MCA-directed photoactivation (1 mm in diameter). Arrow indicates the direction of MCA blood flow. (B) The blood flow in the ipsilateral MCA-supplying territory dropped at 5 hours after photoactivation, but steadily recovered to ∼50% at 1 day (*P < .05 compared with the 5-hour postphotoactivation value by 1-way analysis of variance), ∼65% at 3 days (***P < .001 compared with the 5-hour value by 1-way analysis of variance), and ∼80% at both 5 and 7 days (***) in both RB-PTS and T+RB-PTS models (n = 7 in each group).