Increased TLR4 pathway activation in Runx1 KO neutrophils. (A) Representative western blot of IκBα degradation in whole BM over a time course of 0 to 90 minutes (′) of stimulation with 100 ng/mL LPS (n = 3). Bar graphs depict IκBα levels relative to the 0-minute time point for each genotype after normalization to the Cyclophilin B loading control for each sample (mean ± SD, 2-tailed unpaired Student t tests comparing Runx1 KO and control samples at each time point). Representative of 7 experiments. (B) Representative western blot of IκBα degradation in FACS-purified neutrophils (CD11b⁺SiglecF⁻F4/80⁻Ly6G⁺) over a time course of 0 to 60 minutes of stimulation with 100 ng/mL LPS (n = 7 from 5 experiments). *P ≤ .05.