Increased secretion of inflammatory cytokines, chemokines, and growth factors by Runx1 KO BM. (A-E) Absolute quantification by CBA of inflammatory factor levels in the supernatant of whole BM cells stimulated for 8 hours with vehicle or 100 ng/mL LPS. Bar graphs include independent data points. Error bars represent mean ± standard deviation (SD). Five replicates from 4 experiments were performed for each condition with all results above the limit of detection (blue arrowhead) plotted. For all factors except KC (C), a 2-tailed unpaired Student t test was performed comparing the factor concentration between the control and Runx1 KO LPS-treated samples. Because of limited detection of KC in the control LPS-treated sample (C), a 1-sample Student t test was performed comparing the mean of the Runx1 KO LPS-treated sample to a hypothetical mean = 0. (F-G) Representative FACS plots gated on live singlets and corresponding quantification of the frequencies of monocytes, neutrophils, and eosinophils in the BM (n = 3 from 3 experiments, mean ± SD, 2-tailed unpaired Student t test). Eos, eosinophils; FSC-A, forward scatter area; Mo, monocytes; PE, phycoerythrin. *P ≤ .05; **P ≤ .01.