Coordinate regulation of gene expression by IRF4 and p65 in ATL cells. (A) Heat map depicting the relative mRNA expression levels of genes regulated by IRF4 (left) and p65 (right) on knockdown (KD) of each factor in TL-Om1 cells. A control shRNA (sh-GFP-1) and a shRNA targeting IRF4 (sh-IRF4-1) or p65 (sh-p65) were transduced into TL-Om1 cells in biological duplicates. mRNA was extracted on day 3 posttransduction. Each row corresponds to a gene, and expression values are normalized across each row. (B) Gene set enrichment analysis was conducted to determine the correlation of genes differentially expressed on KD of p65 (top) or IRF4 (bottom). The normalized enrichment scores (NES) and P values are indicated.