IRF4 and NF-κB co-occupy regulatory elements in ATL cells. (A) All regions bound by the IRF4 protein were first identified in TL-Om1 cells. The metagene plot on the left shows the distribution of IRF4 (red) and p65 (blue) ChIP-seq enrichment signals at the IRF4-bound regions (±5 kb from the binding sites) in TL-Om1 cells. The metagene plot on the right shows the levels of H3K27ac enrichment signals within ±3 kp from the center of the IRF4-p65 binding sites in TL-Om1 cells in the 3 groups: bound by IRF4 only (green), bound by p65 only (red), and bound by both IRF4 and p65 (blue). (B) Venn diagram depicting the distribution of the number of genes associated with IRF4 and p65 binding, as determined by ChIP-seq. (C) The density plots on the left show the distribution of the IRF4, p65, and H3K27ac signals for the genes bound by both IRF4 and p65 in TL-Om1 cells and of the H3K27ac signals in various primary ATL samples (ATL1-10). (D-E) Pie charts showing the percentage of IRF (D) and p65 (E) bound genes located in typical enhancers and super-enhancers. (F) The top 5 DNA binding motifs significantly enriched in transcription factor binding regions in each group are shown with P values.