scRNA-seq analysis of peripheral blood cells of patient 7 before and after nivolumab infusions. (A) Top, Violin plots showing the expressions of IFNG, GZMB, and LAG3 in CD8 T cells at different time points. Bottom, Pie charts showing the ratios of IFNG, GZMB, and LAG3 positive CD8 T cells at different time points. (B) Expression levels of PDCD1 projected on T-distributed stochastic neighbor embedding graphs of T₀ and T₁ peripheral blood cells. (C-D) Regulatory networks of signature genes in CD8 T cells at T₀ (C) and T₁ (D), analyzed with ClusterProfiler. Sizes of circles for enriched pathways indicate the numbers of genes enriched in these pathways and colors of dots for each gene indicate their relative expression levels. (E) Line chart showing intracellular EBV-DNA copy number in NK, B, and T cells. (F) Heatmap showing the expression levels of HLH-related genes in CD8 T cells. (G) Heatmaps showing the expression levels of gene ontology (GO) leukocyte degranulation genes (left) and costimulatory receptors genes (right) in CD8 T cells.