Lineage trees for patient 5, showing the N-gly site status of each subclone. The left panel represents the lineage tree of the first relapse event, whereas the right panel represents part of the lineage tree of the third relapse event. Black nodes represent nodes that are absent of N-gly site found in the major clone. Nodes differing in color to the major clone represent subclones with different N-gly site codon sequences. The 4 nodes split into 2 colors (black and orange) in the right panel represent subclones that are absent for the N-gly site found in the major clone of the third relapse (NLT) event, but are positive for the N-gly site found in the first relapse event (NFS). Shared subclones are highlighted in boxes. The full lineage tree of the third relapse event is provided in supplemental Figure 2B.