JNJ-67571244 kills CD33⁺AML cell lines and activates T cells in vitro. (A) T cells from 6 healthy donors were tested in T-cell redirection assays with the indicated cell lines, and percentage of cytotoxicity was determined by flow cytometry. Mean ± standard deviation is graphed. (B) Median values showing EC₂₀, EC₅₀, and concentration producing 90% of the maximum possible effect (EC₉₀) for the cytotoxicity readout from 6 healthy donors. (C) Similar to panel A but here T-cell activation (CD25 upregulation on CD3⁺ T cells) was measured. (D) Similar to panel B but here median EC₂₀, EC₅₀, and EC₉₀ values are shown for the T-cell activation readout from 6 healthy donors.