Figure 1.
JNJ-67571244 binds to CD33, an antigen abundantly expressed by leukemic blasts. (A) Expression and antigen density of CD33 was evaluated in 49 AML samples. Mean ± standard deviation are graphed. (B) CD33+ and CD33– cell lines were stained for 4 hours with various concentrations of JNJ-67571244 to characterize the surface-binding profiles of the bispecific antibody. Binding of the bispecific antibody was detected by staining with mouse anti-human IgG4 (details provided in the supplemental Methods).