Multiplex PCR of leukemic BM with respective tails from T2ΔT3 and ΔT2T3 mice. Multiplex PCR was performed for the regions spanning the loxP sites on Tet2 (exon 11) and Tet3 (exons 8 and 9) in the BM and tail DNA of T2ΔT3 (n = 9) (A) and ΔT2T3 (n = 8) (B) mice, respectively. Lack of amplification of the Tet2 and Tet3 wild-type allele in BM from T2ΔT3 (n = 9) and ΔT2T3 (n = 6) mice is highlighted with red arrows. Amplification of the Tet3 wild-type allele in BM from ΔT2T3 mice (n = 2; #47 and #60) is highlighted with yellow arrows. flox, del, and wild: PCR products representing floxed, deleted, and wild-type alleles.