An increase in plasmablasts in tumor lymph nodes of Roquin^san/+mice. (A) Pictogram illustrating differences between tumor-free and -bearing mice, as well as providing a pictorial explanation of tumor-free, nontumorous (non-tumor), and tumorous (tumor) lymph nodes. (B-D) Representative flow cytometric analyses and frequencies of B-cell populations using the surface markers Fas and GL7 (n = 10 tumor-free; n = 11 nontumorous; and n = 15 tumorous samples). (E-F) Representative flow cytometry plots and histograms comparing the frequency of plasmablasts (n = 5 tumor-free, n = 6 nontumorous; and n = 9 tumorous samples). (G-I) Expression profile of Bcl6 and IRF4 in GL7^hiFas⁺ B cells as depicted through representative flow cytometry plots and frequencies (n = 5 tumor-free; n = 5 nontumorous; and n = 9 tumorous samples). Error bars in panels C-D,F,H-I represent the standard error of the mean (SEM). Data are pooled from at least 2 independent experiments. *P < .05; **P < .01; ***P < .001; ****P < .0001. ns, not significant.