Procoagulant activity of RBC-MVs assessed using TG or an MV tissue factor activity assay. (A) TG in recalcified MV-free NPP in MVs from the indicated day (D) of storage, from a representative donor sample. (B) TG was performed in NPP in the absence (red) or presence (green) of anti-human tissue factor (HTF1 Ab), anti-human tissue factor-FVIIa complex (5G9 Ab; blue), or MV-free plasma deficient in FVII (def-VII; dashed dark purple). (C) Measurement of tissue factor activity on RBC-MVs. Innovin (0.5 pM) was used as the positive control and buffer as the negative control. Data are presented as the mean ± standard deviation of 3 independent experiments. (D) TG performed in MV-free plasma deficient in FXII (def-XII; green), deficient in FXI (def-XI; dashed dark purple), or deficient in FIX (def-IX; blue), compared with NPP (red). Data are representative of 3 independent experiments.