Antibodies targeting the N-terminal domain of ERFE increase hepcidin expression and ameliorate anemia in thalassemic mice. Four-week-old male Hbb(th3/+) mice were treated intravenously with 5 mg/kg of IgG2A control antibody, or anti-ERFE 15.1, twice a week for 8 weeks. After 8 weeks of treatment, mice were killed for analysis of liver hepcidin messenger RNA expression (A), liver non-heme iron (B), Hamp to liver non-heme iron content (liver iron content [LIC]) ratio (C), serum iron (D), spleen non-heme iron (E), spleen to body weight (F), and blood parameters (G). *P < .05; **P < .01; ***P < .001 using the Mann-Whitney U test. HCT, hematocrit; HGB, hemoglobin; RBC, red blood cells. n = 6, IgG2A group; n = 9, the 15.1 group.