Fig. 7.
Splenic CD8+ DC and CD8− DC from flt3L−/− mice stimulate allogeneic T-cell proliferation in an MLR.

Splenic CD8+ DC and CD8 DC from flt3L−/− mice stimulate allogeneic T-cell proliferation in an MLR.

Splenic CD8α+ DC and CD8α DC from flt3L+/+ and flt3L−/− mice were purified by flow cytometry (see “Materials and methods”), and cultured in titrating numbers with 1 × 105 purified allogeneic naive CD4+ T cells isolated from the spleen and LN of DBA/2 mice. T-cell proliferation as measured by uptake of tritiated thymidine was determined on day 4; ▪ indicates flt3L+/+ CD8αDC; □, flt3L−/− CD8α DC; •, flt3L+/+ CD8α+ DC; ○, flt3L−/− CD8α+ DC. Data are presented as the mean ± SD of triplicate counts. The experiment is representative of 3 separate experiments.

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