EMSA analysis of bacterially expressed GST-TEL2 proteins.

(A) Radiolabeled oligomers encoding the TEL1 CDBS were incubated with eluates of either GST only, GST-T2, or GST-T2ETS proteins. DNA-protein complexes were supershifted with α-T2. Combinations of DNA, protein, and aby are indicated above each lane. The GST-T2+DNA supershift (top of figure) and the GST-T2ETS supershift are denoted by arrows labeled “SS.” A complex associated with the addition of α-T2 antisera is denoted with an “*.” (B) GST-T2 protein was incubated with radiolabeled-TEL1 CDBS and increasing amounts of unlabeled TEL1 CDBS. The molar excess of unlabeled probe added to each binding reaction is shown above each lane. Complexed and free probe were separated by PAGE and detected by autoradiography. The GST-T2ETS protein + CDBS probe complex is indicated with an arrow labeled “P+P.” Uncomplexed probe is shown at the bottom of the figure by the “Probe”-labeled arrow.